Conan, Ran and Sonoko run into Hondo Eisuke at a hospital as he is searching for Mizunashi Rena by showing nurses a picture of his older sister. He tells Ran and Sonoko that she ran away 10 years ago and is looking for her. Conan knows he's actually looking specifically for Mizunashi Rena who was hurt and put in the hospital during the confrontation with the men in black, but he isn't sure why.
Sonoko sees the picture and says that she (the girl in the picture, Eisuke's older sister) looks like Mizunashi Rena. However Eisuke keeps insisting that she isn't. They check an old news broadcast that Mizunashi was in to compare to the picture.
When Eisuke was a kid he got hurt and got a blood transfusion from his sister. She said he is lucky because he has a type of blood that anyone can receive, so he should be careful. In other words he has the blood type that anyone can receive in a blood transfusion, type O. And since type O people can give to anyone but can only receive from other type O people that means that his older sister is also a type O person. However, Mizunashi Rena, in the video that they were checking to compare to the photo, states that she has type AB (because she is reporting at a place where many people got hurt by an explosion and nurses where looking for blood donors).
So Conan knows that Mizunashi Rena and Hondo Eisuke's older sister are different people.(Later it turns out that they actually are the same person because of something he finds out about Eisuke.) But that means that the reason Hondo Eisuke is looking for Mizunashi Rena is possibly because he thinks someone had her killed and took her place. The reason they did that probably has some connection to Hondo Eisuke's dad, a member of "The Company".
From what Eisuke tells them they then find out that as a kid Eisuke lived with his mother while she was a maid a a rich person's house. At that time his sister was studying abroad, but when she returned home on break she would live there with them. But his father live in Osaka for his job and he only saw him on New Year's. Then his mother died of an illness and he lived for a short while with his father in Osaka. But now, it seems that he's oversees on business. Eisuke now lives with a friend of his dad. His dad sends him a monthly allowance.
Eisuke needs to change his clothes at one point and without thinking starts to change in front of Ran. He says he was used to it because he always used to in front of his sister when he was little. Conan notices something at that point and tells Haibara that it seems he's hiding something else. That is in connection with why Conan knows that Rena really is his sister
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